منابع مشابه
Enzymatic activity of Nitrosomonas extracts.
The sole energy-yielding reaction of the autotrophic nitrifying bacterium Nitrosomonas europaea involves the conversion of ammonium to nmtrite. As early as 1926, Kluyver and Donker postulated that the six electron oxidation proceeds with the intermediary formation of hydroxylamine and hyponitrous acid. Only recently have there been any studies of the biochemical mechanism of chemoautotrophic am...
متن کاملMethane oxidation by Nitrosomonas europaea.
Methane inhibited NH4+ utilization by Nitrosomonas europaea with a Ki of 2mM. O2 consumption was not inhibited. In the absence of NH4+, or with hydrazine as reductant, methane caused nearly a doubling in the rate of O2 uptake. The stimulation was abolished by allylthiourea, a sensitive inhibitor of the oxidation of NH4+. Analysis revealed that methanol was being formed in these experiments, wit...
متن کاملIsolation of Nitrosomonas in pure culture.
Although it has been 68 years since Winogradsky (1890) accomplished the first isolation of nitrifying bacteria, the methods employed have not been improved significantly, and pure cultures remain incredibly difficult to obtain. The literature records numerous attempts to secure Nitrosomonas in pure culture. Nevertheless, the organism has seldom been isolated by dilution techniques. In the relat...
متن کاملPhosphorylation by extracts of Nitrosomonas europaea.
Burge, W. D. (University of California, Berkeley), E. Malavolta, and C. C. Delwiche. Phosphorylation by extracts of Nitrosomonas europaea. J. Bacteriol. 85:106-110. 1963.-Cellfree preparations of Nitrosomonas europaea are capable of oxidizing hydroxylamine, but not ammonium ion, to nitrite. The quantity of nitrite formed by our preparations was, at most, equivalent to only 70% of the hydroxylam...
متن کاملEnzyme Immunoassay Detection of Nitrosomonas europaea.
An exploratory effort to selectively detect the presence of a nitrifying bacterium, Nitrosomonas europaea, successfully demonstrated the fundamental utility of an enzyme-based immunoassay protocol. The applied polyclonal antibody test seemingly offered a marked improvement over the available analytical options, including plating, activity, and fluorescence immunoassay techniques. Following an i...
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ژورنال
عنوان ژورنال: Nature
سال: 1957
ISSN: 0028-0836,1476-4687
DOI: 10.1038/179789a0